QUANTITATIVE METHOD FOR DETECTING YESSOTOXINS IN FISH PRODUCTS BASED ON THE ACTIVATION CAUSED BY THE TOXIN IN CELLULAR PHOSPHODIESTERASE AND THERAPEUTIC USEFULNESS OF SAID ACTIVATION
Application number: | PCT/ES2004/000343 (21.07.2004) E04742071 (21.07.2004)
| Priority number: | ES20030001773 (25.07.2003)
| Applicant: | UNIVERSIDADE DE SANTIAGO DE COMPOSTELA (ES) CITT - EDIFICIO CACTUS, CAMPUS SUR, 15782 SANTIAGO DE COMPOSTELA | Inventor/s: | BOTANA LOPEZ, L.M. (ES); ALFONSO RANCANO, A. (ES); PAZOS GULDRIS, M.J. (ES); RODRIGEUZ VIEYTES, M. (ES); LOZA GARCIA, M.I. (ES); VIEITES BAPTISTA DE SOUSA, Juan Manuel (ES);
| Abstract: | The invention relates to a quantitative method for detecting yessotoxins in fish products based on the activation caused by the toxin in cellular phosphodiesterase and therapeutic usefulness of said activation. The cellular target of yessotoxin (YTX) and analogues is the activation of phosphodiesterases (PDEs). The PDEs-YTX union emits a measurable signal. The union can be quantified by means of an affinity biosensor or fluorescence. The biosensor detects bimolecular interactions and makes it possible to determine the presence of YTX due to its interaction with PDEs. Plate fluorescence makes it possible to determine variations in the speed of degradation of fluorescent derivative anthranyloil-AMPc. The speed with which the PDEs degrade this molecule increases in the presence of YTX. The YTX inhibits the immunological activation of rat mastocytese and induces a cytotoxic effect in human hepatocarcinoma cells, which provides two therapeutic utilities of YTXs as anti-allergic and anti-tumoral compound.
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